Like humans, plants are also come under the category of living beings. They contain cells and tissues to facilitate their growth and development along with the transportation of water and minerals. The transporting tissues in plants are xylems and phloem that are present in all parts. These tissues and their placement can easily be understand through this activity of preparing the transverse section of monocot and dicot stem and roots.
Your Total Savings ₹14333 What is the aim of studying the transverse section of dicot and monocot roots and stems?
The aim is to prepare a temporary stained mount of a transverse section of dicot and monocot stem and root to study various plant tissues.
What materials are required for this study?
Materials required include preserved material of sunflower and maize root and stem, microscope, sharp blade, slides, watch glass, coverslips, safranin, brush, glycerine, and blotting paper.
What is the procedure for preparing the sections?
The plant material is held and sliced into thin sections. These sections are then shifted into a watch glass with water.
What is the procedure for staining the sections?
The thin sections are shifted to a watch glass holding safranin stain and left to rest for a couple of minutes. The sections are then drained of the stain and rinsed with water.
What are the precautionary measures to be taken during this study?
Ensure adequate water supply while dissecting the section, use a brush while working with sections, place the coverslip gently to avoid air bubbles, and remove excess glycerine with filter paper.