In C4 plants, the primary CO2 acceptor is: 

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  1. Phosphoenol pyruvate
  2. 3-phosphoglyceric acid
  3. RUBICO
  4. Oxaloacetic acid

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Option 1 : Phosphoenol pyruvate
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The correct answer is Phosphoenol pyruvate

Concept:

Different Plants use different photosynthetic processes for atmospheric carbon fixation.

The three main processes are:

C3 cycle - 

  • ​In the C3 pathway or Calvin cycle, the primary CO2 acceptor molecule is ribulose bisphosphate (RuBP)
  • This reaction is catalysed by the enzyme RuBP carboxylase which results in the formation of two molecules of 3-PGA.

C4 cycle - 

  • The primary CO2 acceptor is 3-carbon molecule phosphoenolpyruvate (PEP) and is present in the mesophyll cells.
  • The enzyme responsible for this fixation is PEP carboxylase or PEPcase

CAM pathway - 

  • The primary CO2 acceptor is PEP at nightRuBP in the day. 
  • The enzyme responsible for this fixation is PEPcase at nightRubisco at daytime.
Points C3 Pathway C4 Pathway CAM Pathway

Initial CO2 acceptor

Ribulose-1, 5-bisphosphate (RuBP)

Phosphoenolpyruvate (PEP)

PEP at night, RuBP in the day

CO2-fixing enzyme

Ribulose-1, 5-bisphosphate  carboxylase/oxygenase(RuBisCo)

Phosphoenolpyruvate carboxylase (PEPcase) then Rubisco

PEPcase at night, Rubisco at daytime

First stable product of CO2 fixation

3-phosphoglycerate (3-PGA)

Oxaloacetate (OAA) in C4 cycle

OAA at night, 3-PGA at daytime

Explanation:

Phosphoenolpyruvate (PEP):

  • PEP is the primary CO2 acceptor in C4 plants, occurring in the mesophyll cells.
  • The enzyme PEP carboxylase catalyzes the fixation of CO₂ to PEP, forming oxaloacetate, which is then converted to malate or aspartate.
  • This initial fixation step is highly efficient, even at low CO₂ concentrations, contributing to the high photosynthetic efficiency of C4 plants.
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